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Streptomyces phages
Life Domain
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This host-range assay was performed by Aël Hardy, a PhD student in the laboratory of Julia Frunzke (Forschungszentrum Jülich,
The method used was spotting assay, with serial dilutions of the phage solutions being spotted on a bacterial lawn propagated by mixing 200 µl of Streptomyces overnight culture with 4 ml top agar. Liquid cultures of Streptomyces was performed in Glucose Yeast Medium (GYM) medium - to which glass beads were added to favor dispersed growth - for all species except S. coelicolor which was grown in Yeast Extract Malt Extract (YEME) medium to ensure dispersion. For double agar overlays, GYM agar was used for all species, with 0.5% and 1.5% agar for the top and bottom layers, respectively. Temperature of cultivation was 30°C.
The viruses used in the study were all isolated from forest soil surrounding the Forschungszentrum Jülich. The outcome of the spot assays is reported as follows: plaque formation (2), clearance of the bacterial lawn without visible plaques (1), no plaque or lysis visible (0).
Provided By
Aël Hardy
Creation Date
July 26, 2023, 4:10 p.m.
Last Edition Date
Aug. 1, 2023, 5:36 p.m.
Viruses 5
Hosts 11
Responses 55
Response scheme used
Original response Response mapped
0.0 No infection
1.0 Intermediate
2.0 Infection