Data Source Explore it

Information

Name: Streptomyces phages
Life Domain: bacteria
Publication: Open it
Description: This host-range assay was performed by Aël Hardy, a PhD student in the laboratory of Julia Frunzke (Forschungszentrum Jülich, j.frunzke@fz-juelich.de).
The method used was spotting assay, with serial dilutions of the phage solutions being spotted on a bacterial lawn propagated by mixing 200 µl of Streptomyces overnight culture with 4 ml top agar. Liquid cultures of Streptomyces was performed in Glucose Yeast Medium (GYM) medium - to which glass beads were added to favor dispersed growth - for all species except S. coelicolor which was grown in Yeast Extract Malt Extract (YEME) medium to ensure dispersion. For double agar overlays, GYM agar was used for all species, with 0.5% and 1.5% agar for the top and bottom layers, respectively. Temperature of cultivation was 30°C.
The viruses used in the study were all isolated from forest soil surrounding the Forschungszentrum Jülich. The outcome of the spot assays is reported as follows: plaque formation (2), clearance of the bacterial lawn without visible plaques (1), no plaque or lysis visible (0).
Visibility: Public
Provided By: Aël Hardy
Creation Date: July 26, 2023, 4:10 p.m.
Last Edition Date: Aug. 1, 2023, 5:36 p.m.

Content

Viruses	5
Hosts	11
Responses	55

Download it

The file to fill is a double-entry table (see example on the right) where rows are viruses and columns are hosts, and for which the infection status is entered as a number. The recommended values to use are: 0 ( No infection), 1 ( Intermediate) and 2 ( Infection), but you will later be able to map any value used to this global scheme.

More information can be found in the documentation.

	E. coli MG1655 (NC_000913.3)	E. coli O157:H7 (AE005174.2)
T4 (NC_000866.4; HER:27)	2	0
T7 (NC_001604.1)	1	2
MyVirus	2	1

download example file

Response scheme used

Original response	Response mapped
0.0	No infection
1.0	Intermediate
2.0	Infection

Viruses

Alderaan(MT711975; TAX:2739833), Coruscant(MT711976; TAX:2739834), Dagobah(MT711977; TAX:2740180), Endor1(MT711978; TAX:2740181), Endor2(MT711979; TAX:2740182)

Hosts

Streptomyces coelicolor M145, Streptomyces coelicolor M600, Streptomyces cyaneofuscatus(DSM 40148), Streptomyces griseus(DSM 40236; TAX:1911), Streptomyces lividans TK24(NZ_CP009124; TAX:457428), Streptomyces olivaceus(DSM 41536), Streptomyces platensis(DSM 40041), Streptomyces rimosus subsp. rimosus(DSM 40260), Streptomyces scabiei(DSM 41658), Streptomyces venezuelae ATCC 10712(NC_018750.1; TAX:953739), Streptomyces xanthochromogenes(DSM 40111)