Data Source Explore it

Information

Name: S. epidermidis phages against human mastitis and healthy strains
Life Domain: bacteria
Publication: Open it
Description: Method: 5 µL of concentrated phage lysate (10^9 PFU/mL) was dropped onto a TSB plate overlaid with S. epidermidis (10^8 CFU/mL). The host range was confirmed by theplaque assay: 0.1 mL of stationary-phase host culture (10^8 CFU/mL) was mixed with several dilutions of individual phage suspensions in 3 ml of molten TSB top agar and the mixture was poured on TSA plates. Efficiency of plaque formation (EOP) of selected phages was determined by dividing the phage titer on the test strain by the phage titer on the reference strain S. epidermidis F12. This strain was selected because it is infected by all the isolated phages. Look at Table 1 of article for EOP values.
The strains are Staphylococcus epidermidis strains, which are either coming from human mastitis samples or healthy women (see Table 1 of article).
The bacteriophages were isolated by mytomycin C induction of S. epidermidis strains.
The intermediate value was used to represent "inhibition halo" phenotype.
Visibility: Public
Provided By: Pilar García
Creation Date: May 25, 2021, 12:41 p.m.
Last Edition Date: May 25, 2021, 12:41 p.m.

Content

Viruses	3
Hosts	65
Responses	195

Download it

The file to fill is a double-entry table (see example on the right) where rows are viruses and columns are hosts, and for which the infection status is entered as a number. The recommended values to use are: 0 ( No infection), 1 ( Intermediate) and 2 ( Infection), but you will later be able to map any value used to this global scheme.

More information can be found in the documentation.

	E. coli MG1655 (NC_000913.3)	E. coli O157:H7 (AE005174.2)
T4 (NC_000866.4; HER:27)	2	0
T7 (NC_001604.1)	1	2
MyVirus	2	1

download example file

Response scheme used

Original response	Response mapped
0.0	No infection
0.0000000000000001	Intermediate
0.000000374	Infection
0.000000641	Infection
0.00000104	Infection
0.00000115	Infection
0.00000506	Infection
0.00000631	Infection
0.00000633	Infection
0.000428	Infection
0.32	Infection
0.46	Infection
0.47	Infection
0.67	Infection
0.99	Infection
1.0	Infection
1.02	Infection
1.07	Infection
1.93	Infection
4.03	Infection

Viruses

phiIPLA5(NC_018281; TAX:1197952), phiIPLA6, phiIPLA7(NC_018284; TAX:1197953)

Hosts

S. epidermidis 4GLI4, S. epidermidis AEA1, S. epidermidis AQLD3, S. epidermidis AQLI2, S. epidermidis ARLI1, S. epidermidis ASLD1, S. epidermidis ASLD2, S. epidermidis ASLD3, S. epidermidis ASLI3, S. epidermidis B, S. epidermidis B1CD2, S. epidermidis C213, S. epidermidis CJ11, S. epidermidis CJ9, S. epidermidis CJBP1, S. epidermidis CJBP2, S. epidermidis CJBP3, S. epidermidis CJBP3.1, S. epidermidis D623, S. epidermidis DC2LAe, S. epidermidis DD2Laa, S. epidermidis DF2Lbk, S. epidermidis DG2ñ, S. epidermidis DH3LIK, S. epidermidis F12, S. epidermidis K, S. epidermidis LCC5081, S. epidermidis LCC5092, S. epidermidis LCO16, S. epidermidis LCO17, S. epidermidis LEO10, S. epidermidis LEO11, S. epidermidis LEO35, S. epidermidis LG005, S. epidermidis LG006, S. epidermidis LG5082a, S. epidermidis LI5081, S. epidermidis LO5081, S. epidermidis LO5082, S. epidermidis LO5RB1, S. epidermidis LP222, S. epidermidis LP223, S. epidermidis LV221, S. epidermidis LV222, S. epidermidis LV5081, S. epidermidis LV521, S. epidermidis LV5RB3, S. epidermidis LX5081, S. epidermidis LX5RB3, S. epidermidis LX5RB4, S. epidermidis M121, S. epidermidis P2LD1, S. epidermidis PLD22, S. epidermidis S1LDC13, S. epidermidis S1LDC18, S. epidermidis SILDC13, S. epidermidis V1LD1, S. epidermidis YLIC13, S. epidermidis YLIC14, S. epidermidis YLIC16, S. epidermidis YLIC17, S. epidermidis Z2LDC11, S. epidermidis Z2LDC12, S. epidermidis Z2LDC14, S. epidermidis Z2LDC17