Data Source Explore it

Information

Name: Clostridium difficile phages
Life Domain: bacteria
Publication: Open it
Description: The experiments were realized by Ognjen Sekulovic, Julian R. Garneau, and Audrey Néron in Pr. Louis-Charles Fortier’s lab.

5 uL volumes of undiluted and 1/100 diluted phage lysates (10e7 to 10e9 PFU/mL) were deposited on top of soft agar overlays containing log-phase cultures of C. difficile test strains. The selected strain panel comprised major PCR ribotypes like 001, 014, 027, and 078, as well as other ribotypes from various origins (humans, animals, environment). The intensities of the clearing zones were recorded.

Results were published in :
Characterization of Temperate Phages Infecting Clostridium difficile Isolates of Human and Animal Origins, Ognjen Sekulovic, Julian R. Garneau, Audrey Néron, Louis-Charles Fortier, Applied and Environmental Microbiology, 2014. (PMID: 24532062) (DOI: 10.1128/AEM.00237-14)
Visibility: Public
Provided By: Julian Garneau
Creation Date: March 13, 2021, 5:07 p.m.
Last Edition Date: March 17, 2021, 5:10 p.m.

Content

Viruses	15
Hosts	37
Responses	555

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The file to fill is a double-entry table (see example on the right) where rows are viruses and columns are hosts, and for which the infection status is entered as a number. The recommended values to use are: 0 ( No infection), 1 ( Intermediate) and 2 ( Infection), but you will later be able to map any value used to this global scheme.

More information can be found in the documentation.

	E. coli MG1655 (NC_000913.3)	E. coli O157:H7 (AE005174.2)
T4 (NC_000866.4; HER:27)	2	0
T7 (NC_001604.1)	1	2
MyVirus	2	1

download example file

Response scheme used

Original response	Response mapped
0.0	No infection
1.0	Intermediate
2.0	Infection

Viruses

phiCD111(NC_028905; TAX:1582150), phiCD146(NC_028958; TAX:1582151), phiCD24-2, phiCD38-2(NC_015568; TAX:1032362), phiCD481-1(NC_028951; TAX:1582153), phiCD481-2, phiCD505(NC_028764; TAX:1582154), phiCD506(NC_028838; TAX:1582155), phiCD508, phiCD52(GCA_001369435), phiCD526, phiMMP01(NC_028883; TAX:1582156), phiMMP02(NC_019421; TAX:1204534), phiMMP03(NC_028959; TAX:1582157), phiMMP04(NC_019422; TAX:1204535)

Hosts

CD105, CD111, CD117, CD118, CD125, CD19, CD192, CD211, CD24, CD273, CD274, CD316, CD326, CD337, CD383, CD384, CD398, CD419, CD420(NC_013315; TAX:645462), CD425(FN668944; TAX:699032), CD426, CD427, CD428, CD429, CD430, CD475, CD481, CD490, CD493, CD505, CD511, CD515, CD540, CD544, CD73, CD77, CD93