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Information

Name: 11 Phages host range against 32 Phytopathogen Pseudomonas syringae pathovar phaseolicola and pathovar actinidiae
Life Domain: bacteria
Publication: Open it
Description: The experiment was realized by Gabriele Martino and Marina Ciuffo (gabriele.martino@ipsp.cnr.it, marina.ciuffo@ipsp.cnr.it).Bacteriophages were originally isolated using Pseudomonas syringae pv. actinidiae (Psa)
strain K7#8 and Pseudomonas syringae pv. phaseolicola (Pph)
strain Cuneo
#6_18, isolated from symptomatic plants. The host range of each selected phage was tested through an efficiency of plating
(EOP) analysis. Serially diluted phage lysates were applied (5 ul drops) to soft agar
containing one of the bacteria strains of our collection (4 mL of melted 0.6% LBLS soft-agar
and 250 ul of an overnight culture of Psa or Pph). For each bacteria phage combination
three different dilutions were spotted from the same phage crude lysate.
The plates were incubated at 26 °C for 24 h and then the plaques were counted to
obtain phage titration. For each phage, all the titration values were compared quantitatively
to the titration value of the same phage infecting the strain used for its isolation.
Visibility: Public
Owner: Gabriele Martino Contact owner
Creation Date: Dec. 29, 2021, 11:39 a.m.
Last Edition Date: Jan. 13, 2022, 5:05 p.m.

Content

Viruses	11
Hosts	32
Responses	352

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The file to fill is a double-entry table (see example on the right) where rows are viruses and columns are hosts, and for which the infection status is entered as a number. The recommended values to use are: 0 ( No infection), 1 ( Intermediate) and 2 ( Infection), but you will later be able to map any value used to this global scheme.

More information can be found in the documentation.

	E. coli MG1655 (NC_000913.3)	E. coli O157:H7 (AE005174.2)
T4 (NC_000866.4; HER:27)	2	0
T7 (NC_001604.1)	1	2
MyVirus	2	1

download example file

Response scheme used

Original response	Response mapped
0.0	No infection
1.0	Intermediate
2.0	Infection

Viruses

MR8(MT104471; TAX:2711175), pphageB1(MT354570; TAX:2755402), pphageB21(MZ868714; TAX:2875712), pphageBV72(MZ868717; TAX:2875718), pphageT12(MZ868716; TAX:2875713), pphageT21(MZ868715; TAX:2875714), psageA1(MT740307; TAX:2759194), psageB1(MT354569; TAX:2755401), psageB2(MZ348425; TAX:2859562), psageK4(MZ348426; TAX:2859563), psageK4e(MZ868713; TAX:2875723)

Hosts

Pph #13, Pph #14, Pph #9, Pph Cuneo, Pph Cuneo #13, Pph Cuneo #6_17, Pph Cuneo #6_18, Pph Cuneo #7, Pph PSS, Psa #298a, Psa #391a, Psa #392a, Psa #453d, Psa #453e, Psa #454d, Psa #454e, Psa #509c, Psa #509d, Psa #509e, Psa #5712.19, Psa #5726.19, Psa #5747.19, Psa #5846.19, Psa #5847.19, Psa #5850.19, Psa K4#10, Psa K4#3, Psa K4#6, Psa K4#7, Psa K4#9, Psa K7#1, Psa K7#8