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11 Phages host range against 32 Phytopathogen Pseudomonas syringae pathovar phaseolicola and pathovar actinidiae
Life Domain
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The experiment was realized by Gabriele Martino and Marina Ciuffo (, were originally isolated using Pseudomonas syringae pv. actinidiae (Psa)
strain K7#8 and Pseudomonas syringae pv. phaseolicola (Pph)
strain Cuneo
#6_18, isolated from symptomatic plants. The host range of each selected phage was tested through an efficiency of plating
(EOP) analysis. Serially diluted phage lysates were applied (5 ul drops) to soft agar
containing one of the bacteria strains of our collection (4 mL of melted 0.6% LBLS soft-agar
and 250 ul of an overnight culture of Psa or Pph). For each bacteria phage combination
three different dilutions were spotted from the same phage crude lysate.
The plates were incubated at 26 °C for 24 h and then the plaques were counted to
obtain phage titration. For each phage, all the titration values were compared quantitatively
to the titration value of the same phage infecting the strain used for its isolation.
Gabriele Martino Contact owner
Creation Date
Dec. 29, 2021, 11:39 a.m.
Last Edition Date
Jan. 13, 2022, 5:05 p.m.
Viruses 11
Hosts 32
Responses 352
Response scheme used
Original response Response mapped
0.0 No infection
1.0 Intermediate
2.0 Infection