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Information
Name
Host range of 3 phapecoctavirus on avian pathogenic Escherichia coli strains (APEC) of various serogroup
Life Domain
bacteria
Description
The experiment was realized by Catherine Schouler in the team "pathogenesis of avian colibacillosis" of UMR1282 Infectiology and Public Health (catherine.schouler@inrae.fr). It was a spotting method, on which 10 µL of undiluted lysate was spotted on a lawn of bacteria grown to mid-exponantial phase (LB medium Miller formula). The results were assessed visually by the presence of a lysis zone. If this one was clear, result was scored 2, absence of lysis zone was scored 0, a more or less clear zone was scored 1. Phages vB_EcoM_ ESCO37 and vB_EcoM_ ESCO5 were isolated by us from chicken ceacal content on Escherichia coli strains. Phage vB_EcoM_ ESCO37-5 resulted from a recombination between genome of phages vB_EcoM_ ESCO37 and vB_EcoM_ ESCO5. Those three phages were tested on a collection of avian pathogenic E. coli strains of various serogroup.
Visibility
Public
Owner
Catherine Schouler Contact owner
Creation Date
March 12, 2020, 1:39 p.m.
Last Edition Date
March 17, 2021, 2:58 p.m.
Content
Viruses 3
Hosts 56
Responses 168
Response scheme used
Original response Response mapped
0.0 No infection
1.0 Intermediate
2.0 Infection