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Information

Name: Tests of E. coli phages from D'Hérelle collection on E. coli isolates from infant fecal samples
Life Domain: bacteria
Publication: Open it
Description: Host range tests performed by Aurélie Mathieu, in Marie-Agnès Petit's lab. Technique : 1 mL of overnight culture was washed over the surface of a squared plate of dried LB supplemented with 5 mM CaCl2, 10 mM MgSO4 and 0.2% maltose. 5 μl of filtered supernatants containing coliphages or purified coliphage stocks were applied on plates inoculated with the strain of interest. Plates were incubated overnight at 37 °C. The interactions were classified as either positive (with clear or turbid lysis plaques or confluent lysis spots) or negative (no lysis spots and no lysis plaques). The coliphages are all coming from the D'Hérelle collection. The E. coli strains were isolated from infant fecal samples. Those results were published in Nature Communications, 2020, Mathieu.
Visibility: Public
Owner: Marie-Agnès Petit Contact owner
Creation Date: March 24, 2020, 5:08 p.m.
Last Edition Date: Jan. 21, 2021, 7:55 a.m.

Content

Viruses	16
Hosts	84
Responses	1344

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The file to fill is a double-entry table (see example on the right) where rows are viruses and columns are hosts, and for which the infection status is entered as a number. The recommended values to use are: 0 ( No infection), 1 ( Intermediate) and 2 ( Infection), but you will later be able to map any value used to this global scheme.

More information can be found in the documentation.

	E. coli MG1655 (NC_000913.3)	E. coli O157:H7 (AE005174.2)
T4 (NC_000866.4; HER:27)	2	0
T7 (NC_001604.1)	1	2
MyVirus	2	1

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Response scheme used

Original response	Response mapped
0.0	No infection
1.0	Infection

Viruses

1(KP869100; TAX:1508671; HER:255), 121Q(NC_025447; TAX:1555202; HER:128), 4(KP869102; TAX:1508679; HER:258), 8(KP869106; TAX:1508683; HER:262), I2-2(NC_001332; TAX:10869; HER:213), MS2(NC_001417; TAX:12022; HER:462), N4(NC_008720; TAX:10752; HER:119952179), PR772(AY441783; TAX:261665; HER:222), T1(NC_005833.1; TAX:1921008; HER:24), T3(NC_003298.1; TAX:10759; HER:26), T4(NC_000866.4; TAX:10665; HER:27), T5(NC_005859.1; TAX:10726; HER:28), T7(NC_001604.1; TAX:10760; HER:30), λvir(NC_001416; TAX:10710; HER:37), ΦX174(NC_001422; TAX:10847; HER:36), Ω8(HER:315)

Hosts

6A1, 6A2, 6A3, 6A4, 6A5, 6A6, 6A8, 8A1, 8A10, 8A11, 8A12, 8A2, 8A3, 8A4, 8A5, 8A6, 8A7, 8A8, 8A9, 8C1, 8C10, 8C11, 8C2, 8C3, 8C4, 8C5, 8C6, 8C7, 8C8, 8C9, 8E1, 8E10, 8E12, 8E4, 8E5, 8E6, 8E7, 8E8, 8E9, 8G1, 8G11, 8G12, 8G2, 8G3, 8G4, 8G5, 8G6, 8G7, 8G8, 8G9, 9A1, 9A10, 9A4, 9A5, 9A6, 9A7, 9A8, 9A9, 9C1, 9C10, 9C11, 9C12, 9C2, 9C3, 9C5, 9C7, 9C9, 9E1, 9E10, 9E11, 9E3, 9E4, 9E6, 9E7, 9E8, 9F12, 9G1, 9G2, 9G3, 9G5, 9G7, 9G9, 9H12, 9H5