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Tests of E. coli phages from D'Hérelle collection on E. coli isolates from infant fecal samples
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Host range tests performed by Aurélie Mathieu, in Marie-Agnès Petit's lab. Technique : 1 mL of overnight culture was washed over the surface of a squared plate of dried LB supplemented with 5 mM CaCl2, 10 mM MgSO4 and 0.2% maltose. 5 μl of filtered supernatants containing coliphages or purified coliphage stocks were applied on plates inoculated with the strain of interest. Plates were incubated overnight at 37 °C. The interactions were classified as either positive (with clear or turbid lysis plaques or confluent lysis spots) or negative (no lysis spots and no lysis plaques). The coliphages are all coming from the D'Hérelle collection. The E. coli strains were isolated from infant fecal samples. Those results were published in Nature Communications, 2020, Mathieu.
Marie-Agnès Petit Contact owner
Creation Date
March 24, 2020, 5:08 p.m.
Last Edition Date
Jan. 21, 2021, 7:55 a.m.
Viruses 16
Hosts 84
Responses 1344
Response scheme used
Original response Response mapped
0.0 No infection
1.0 Infection